ARG80959

Human ACE2 ELISA Kit

Human ACE2 ELISA Kit for ELISA and Human

Cell Biology and Cellular Response kit

Component

Cat No Component Name Package Temp
ARG80959-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80959-002 Standard 2 X 10 ng/vial 4°C
ARG80959-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG80959-004 Antibody conjugate concentrate (100X) 1 vial (130 µl) 4°C
ARG80959-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG80959-006 HRP-Streptavidin concentrate (100X) 1 vial (130 µl) 4°C
ARG80959-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG80959-008 25X Wash buffer 20 ml 4°C
ARG80959-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG80959-010 STOP solution 10 ml (Ready to use) 4°C
ARG80959-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80959 Human ACE2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ACE2 in serum, plasma (heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name ACE2
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31 pg/ml
Sample Type Serum, plasma (heparin) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Precision Intra-Assay CV: 5.0%
Inter-Assay CV: 5.7%
Alternate Names Angiotensin-converting enzyme homolog; ACEH; Angiotensin-converting enzyme 2; Metalloprotease MPROT15; ACE-related carboxypeptidase; EC 3.4.17.23

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 59272 Human ACE2

Swiss-port # Q9BYF1 Human Angiotensin-converting enzyme 2

Gene Symbol ACE2
Gene Full Name angiotensin I converting enzyme 2
Background The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]
Function Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. [UniProt]
Highlight Related products:
ACE2 antibodies; ACE2 ELISA Kits; ACE2 recombinant proteins;
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ACE2, receptor of 2019-nCoV
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Cell Biology and Cellular Response kit
PTM N-glycosylation on Asn-90 may limit SARS infectivity.
Proteolytic cleavage by ADAM17 generates a secreted form. Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1.

Images (1) Click the Picture to Zoom In

  • ARG80959 Human ACE2 ELISA Kit standard curve image

    ARG80959 Human ACE2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG80959 Human ACE2 ELISA Kit User's manual download icon Download