ARG83836
Human ACSL4 / FACL4 ELISA Kit
Human ACSL4 / FACL4 ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83836 Human ACSL4 / FACL4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ACSL4 / FACL4 in tissue homogenates, cell lysates and other biological fluids |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | ACSL4 / FACL4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.052 ng/ml |
| Sample Type | Tissue homogenates, cell lysates and other biological fluids |
| Standard Range | 0.16 - 10 ng/mL |
| Sample Volume | 100 μl |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| Alternate Names | LACS4; Mental Retardation, X-Linked 68; ACS4; Acyl-CoA Synthetase 4; Fatty-Acid-Coenzyme A Ligase, Long-Chain 4; XLID63; Long-Chain Fatty-Acid-Coenzyme A Ligase 4; Long-Chain-Fatty-Acid--CoA Ligase 4; Long-Chain Acyl-CoA Synthetase 4; Lignoceroyl-CoA Synthase; Arachidonate--CoA Ligase; Long-Chain-Fatty-Acid--CoA Ligase; Mental Retardation, X-Linked 63; FACL4; MRX63; MRX68 |
Application Instructions
| Assay Time | 3.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O60488 Human Long-chain-fatty-acid--CoA ligase 4 |
|---|---|
| Gene Symbol | ACSL4 |
| Gene Full Name | Acyl-CoA synthetase long chain family member 4 |
| Background | The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq, Jan 2016] |
| Function | Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. [UniProt] |
| Cellular Localization | Cell membrane; Endoplasmic reticulum; Membrane; Mitochondrion; Mitochondrion outer membrane. [UniProt] |
| PTM | Phosphoprotein. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG83836 Human ACSL4 / FACL4 ELISA Kit standard curve image
ARG83836 Human ACSL4 / FACL4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83836 Human ACSL4 FACL4 ELISA Kit User_manual |
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