ARG83753
Human Adiponectin ELISA Kit
Human Adiponectin ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83753 Human Adiponectin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Adiponectin in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Adiponectin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 50 µl |
| Precision | Intra-Assay CV: <10% Inter-Assay CV: <10% |
| Alternate Names | ADIPOQ; Adiponectin, C1Q And Collagen Domain Containing; Adiponectin; ACRP30; GBP28; ApM1; ACDC; Adipose Most Abundant Gene Transcript 1 Protein; 30 KDa Adipocyte Complement-Related Protein; Adipocyte Complement-Related 30 KDa Protein; Adipocyte, C1q And Collagen Domain-Containing Protein; Adipocyte, C1Q And Collagen Domain Containing; Adipose Most Abundant Gene Transcript 1; Adipose Specific Collagen-Like Factor; Gelatin-Binding Protein 28; Gelatin-Binding Protein; Adiponectin Precursor; ADIPQTL1; APM-1; ApM-1; ADPN; APM1 |
Application Instructions
| Assay Time | 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 4°C, -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ADIPOQ |
| Gene Full Name | Adiponectin, C1Q And Collagen Domain Containing |
| Background | This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010] |
| Function | Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. [Uniprot] |
| PTM | Disulfide bond, Glycoprotein, Hydroxylation. [Uniprot] |
Images (1) Click the Picture to Zoom In
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ARG83753 Human Adiponectin ELISA Kit standard curve image
ARG83753 Human Adiponectin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83753 Human Adiponectin ELISA Kit User manual |
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