ARG83884
Human C5 / Complement 5 ELISA Kit
Human C5 / Complement 5 ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83884 Human C5 / Complement 5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human C5 / Complement 5 in Serum, Plasma, Cell culture supernatants |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | C5 / Complement 5 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 31.25 pg/ml - 2000 pg/ml |
| Sample Volume | 100 μl |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| Alternate Names | ECLZB; CPAMD4; Complement C5; C5D; C5b; C5a; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 |
Application Instructions
| Assay Time | 3.5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | C5 |
| Gene Full Name | complement component 5 |
| Background | This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the C5 alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mutations in this gene cause complement component 5 deficiency, a disease characterized by recurrent bacterial infections. Alternative splicing results in multiple transcript variants. |
| Function | Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Activated downstream of classical, alternative, lectin and GZMK complement pathways. [C5a anaphylatoxin]: Mediator of local inflammatory process released following cleavage by C5 convertase. Acts by binding to its receptor (C5AR1 or C5AR2), activating G protein-coupled receptor signaling and inducing a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation. [Uniprot] |
| PTM | Disulfide bond, Glycoprotein. [Uniprot] |
Images (1) Click the Picture to Zoom In
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ARG83884 Human C5 / Complement 5 ELISA Kit standard curve image
ARG83884 Human C5 / Complement 5 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
