ARG70612
Human CCL14 recombinant protein (Active)
Human CCL14 recombinant protein (Active) for SDS-PAGE
Overview
| Product Description | E. coli expressed, Active Human CCL14 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CCL14 |
| Species | Human |
| A.A. Sequence | Thr22 - Asn93 |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0 - 20 ng/ml. |
| Alternate Names | SY14; HCC-1; Chemokine CC-1/CC-3; HCC-3; SCYL2; CC-1; MCIF; CC-3; Small-inducible cytokine A14; HCC-1/HCC-3; 3-74; HCC-1(1-74); 4-74; 1-74; NCC-2; CKB1; 9-74; C-C motif chemokine 14; NCC2; SCYA14 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 96% (by SDS-PAGE or HPLC). |
| Buffer | 20 mM PB, with 150 mM NaCl (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CCL14 |
|---|---|
| Gene Full Name | chemokine (C-C motif) ligand 14 |
| Background | This gene, chemokine (C-C motif) ligand 14, is one of several CC cytokine genes clustered on 17q11.2. The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Read-through transcripts are also expressed that include exons from the upstream cytokine gene, chemokine (C-C motif) ligand 15, and are represented as GeneID: 348249. [provided by RefSeq, Dec 2009] |
| Function | Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5. [UniProt] |
| PTM | The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood. HCC-1(1-74), but not HCC-1(3-74) and HCC-1(4-74), is partially O-glycosylated; the O-linked glycan consists of one Gal-GalNAc disaccharide, further modified by two N-acetylneuraminic acids. [UniProt] |
