ARG70613
Human CCL15 recombinant protein (Active)
Human CCL15 recombinant protein (Active) for SDS-PAGE
Overview
| Product Description | E. coli expressed, Active Human CCL15 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CCL15 |
| Species | Human |
| A.A. Sequence | Gln22 - Ile133 |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by a chemotaxis bioassay using human T- lymphocytes is in a concentration range of 1.0 - 10 ng/ml |
| Alternate Names | Small-inducible cytokine A15; SCYL3; LKN-1; 22-92; Chemokine CC-2; HMRP-2B; MIP-1 delta; NCC-3; 29-92; C-C motif chemokine 15; SY15; MIP-1D; MRP-2B; MIP-5; Leukotactin-1; NCC3; SCYA15; HCC-2; 25-92; Macrophage inflammatory protein 5; Mrp-2b; LKN1 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 97% (by SDS-PAGE or HPLC). |
| Buffer | 20 mM PB, with 150 mM NaCl (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CCL15 |
|---|---|
| Gene Full Name | chemokine (C-C motif) ligand 15 |
| Background | This gene is located in a cluster of similar genes in the same region of chromosome 17. These genes encode CC cytokines, which are secreted proteins characterized by two adjacent cysteines. The product of this gene is chemotactic for T cells and monocytes, and acts through C-C chemokine receptor type 1 (CCR1). The proprotein is further processed into numerous smaller functional peptides. Naturally-occurring readthrough transcripts occur from this gene into the downstream gene, CCL14 (chemokine (C-C motif) ligand 14). [provided by RefSeq, Jan 2013] |
| Function | Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15. [UniProt] |
