ARG70624
Human CCL20 / MIP3 alpha recombinant protein (Active)
Human CCL20 / MIP3 alpha recombinant protein (Active) for SDS-PAGE
Overview
| Product Description | E. coli expressed, Active Human CCL20 / MIP3 alpha recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CCL20 / MIP3 alpha |
| Species | Human |
| A.A. Sequence | Ala27 - Met96 |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by a chemotaxis bioassay using human T- lymphocytes is in a concentration range of 10 - 50 ng/ml. |
| Alternate Names | ST38; CC chemokine LARC; Exodus; Macrophage inflammatory protein 3 alpha; 2-70; MIP-3a; Beta-chemokine exodus-1; SCYA20; MIP-3-alpha; LARC; MIP3A; 1-64; Small-inducible cytokine A20; 1-67; CKb4; C-C motif chemokine 20; Liver and activation-regulated chemokine |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 97% (by SDS-PAGE or HPLC). |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CCL20 |
|---|---|
| Gene Full Name | chemokine (C-C motif) ligand 20 |
| Background | This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014] |
| Function | Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. [UniProt] |
| PTM | C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes. |
