ARG70621
Human CCL3L1 recombinant protein (Active)
Human CCL3L1 recombinant protein (Active) for SDS-PAGE
Overview
| Product Description | E. coli expressed, Active Human CCL3L1 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CCL3L1 |
| Species | Human |
| A.A. Sequence | Ala24 - Ala93 |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0 - 10 ng/ml. |
| Alternate Names | LD78; 464.2; MIP1AP; SCYA3L; G0S19-2; SCYA3L1; D17S1718; LD78BETA; LD78-beta(1-70) |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 97% (by SDS-PAGE or HPLC). |
| Buffer | 20 mM PB, with 150 mM NaCl (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CCL3L1 |
|---|---|
| Gene Full Name | C-C motif chemokine ligand 3 like 1 |
| Background | This gene is one of several cytokine genes that are clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins that function in inflammatory and immunoregulatory processes. The protein encoded by this gene binds to several chemokine receptors, including chemokine binding protein 2 and chemokine (C-C motif) receptor 5 (CCR5). CCR5 is a co-receptor for HIV, and binding of this protein to CCR5 inhibits HIV entry. The copy number of this gene varies among individuals, where most individuals have one to six copies, and a minority of individuals have zero or more than six copies. There are conflicting reports about copy number variation of this gene and its correlation to disease susceptibility. This record represents one of two copies that are present on the ALT_REF_LOCI_2 alternate haplotype of the GRCh38 human reference genome assembly. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| Function | Chemotactic for lymphocytes and monocytes. Is a ligand for CCR1, CCR3 and CCR5. Is an inhibitor of HIV-1-infection. The processed form LD78-beta(3-70) shows a 20-fold to 30-fold higher chemotactic activity and is a very potent inhibitor of HIV-1-infection. LD78-beta(3-70) is also a ligand for CCR1, CCR3 and CCR5. [UniProt] |
