ARG70622
Human CCL8 / MCP2 recombinant protein (Active)
Human CCL8 / MCP2 recombinant protein (Active) for SDS-PAGE
Overview
| Product Description | E. coli expressed, Active Human CCL8 / MCP2 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CCL8 / MCP2 |
| Species | Human |
| A.A. Sequence | Gln24 - Pro99 |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10 - 100 ng/ml. |
| Alternate Names | SCYA10; MCP2; Small-inducible cytokine A8; 6-76; Monocyte chemotactic protein 2; HC14; SCYA8; C-C motif chemokine 8; MCP-2; Monocyte chemoattractant protein 2 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 97% (by SDS-PAGE or HPLC). |
| Buffer | 20 mM PB, with 150 mMNaCl (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CCL8 |
|---|---|
| Gene Full Name | chemokine (C-C motif) ligand 8 |
| Background | This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection. [provided by RefSeq, Sep 2014] |
| Function | Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. [UniProt] |
| PTM | N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes. |
