ARG70703
Human CD25 recombinant protein (Active) (His-tagged, C-ter)
Human CD25 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | CHO expressed, His-tagged (C-ter) Active Human CD25 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CD25 |
| Species | Human |
| A.A. Sequence | Glu 22 - Cys213 |
| Expression System | CHO |
| Activity | Active |
| Activity Note | Determinedd by its ability to inhibit the IL-2-dependent proliferation of CTLL-2 cells. The ED50 for this effect is less than 1 µg/mL in the presence of 30 ng/mL of recombinant human IL-2. |
| Alternate Names | IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% by SDS-PAGE gel and HPLC analyses. |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL2RA |
|---|---|
| Gene Full Name | interleukin 2 receptor, alpha chain |
| Background | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009] |
| Function | Receptor for interleukin-2. [UniProt] |
