ARG70653
Human CD28 recombinant protein (Active) (His-tagged, C-ter)
Human CD28 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | HEK293 expressed, His-tagged (C-ter) Active Human CD28 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CD28 |
| Species | Human |
| A.A. Sequence | Asn19 - Pro152 |
| Expression System | HEK293 |
| Activity | Active |
| Activity Note | Determinedd by its ability to bind human CD28 Antibody in functional ELISA. Immobilized Recombinant human CD28 Protein can bind human CD28 Antibody. |
| Alternate Names | Tp44; TP44; T-cell-specific surface glycoprotein CD28; CD antigen CD28 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CD28 |
|---|---|
| Gene Full Name | CD28 antigen |
| Background | The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011] |
| Function | Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. [UniProt] |
| PTM | CD40LG induces tyrosine phosphorylation of isoform 3. |
