ARG70802
Human CD30 recombinant protein (Active) (His-tagged, C-ter)
Human CD30 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | HEK293 expressed, His-tagged (C-ter) Active Human CD30 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CD30 |
| Species | Human |
| A.A. Sequence | Met1 - Lys379 |
| Expression System | HEK293 |
| Activity | Active |
| Activity Note | Determinedd by its ability to bind human CD30L in functional ELISA. Immobilized Recombinant human CD30L Protein at 2 μg/mL (100 μL/well) can bind Recombinant Human CD30 Protein. |
| Alternate Names | Tumor necrosis factor receptor superfamily member 8; Ki-1 antigen; CD30; Ki-1; Lymphocyte activation antigen CD30; CD antigen CD30; D1S166E; CD30L receptor |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE); > 90% (by HPLC) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | TNFRSF8 |
|---|---|
| Gene Full Name | tumor necrosis factor receptor superfamily, member 8 |
| Background | CD30 is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Function | CD30 is a receptor for TNFSF8/CD30L (PubMed:8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed:8999898). [UniProt] |
| PTM | Phosphorylated on serine and tyrosine residues. |
