ARG70731
Human CD64 recombinant protein (Active) (His-tagged, C-ter)
Human CD64 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | CHO expressed, His-tagged (C-ter) Active Human CD64 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CD64 |
| Species | Human |
| A.A. Sequence | Gln16 - His292 |
| Expression System | CHO |
| Activity | Active |
| Activity Note | Determinedd by its ability to bind human IgG in functional ELISA. Immobilized Recombinant human IgG at 1 μg/mL (100 μL/well) can bind Recombinant Human FCGR1 Protein. |
| Alternate Names | High affinity immunoglobulin gamma Fc receptor I; CD64; Fc-gamma RIA; CD antigen CD64; FcgammaRIa; FCRI; IgG Fc receptor I; CD64A; Fc-gamma RI; FcRI; IGFR1 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FCGR1A |
|---|---|
| Gene Full Name | Fc fragment of IgG, high affinity Ia, receptor (CD64) |
| Background | CD64 (FcgammaRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding alpha subunit and promiscuous gamma subunit, which is indispensable for tyrosine-based signaling. However, even the alpha subunit can transduce signals leading to cellular effector functions. The isoform FcgammaRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcgammaRIb and FcgammaRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo. |
| Function | High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. [UniProt] |
| PTM | Phosphorylated on serine residues. |
