ARG70741
Human CD73 recombinant protein (Active) (His-tagged, C-ter)
Human CD73 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | CHO expressed, His-tagged (C-ter) Active Human CD73 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CD73 |
| Species | Human |
| A.A. Sequence | Trp 29 - Ser 551 |
| Expression System | CHO |
| Activity | Active |
| Activity Note | Determinedd by its ability to bind mouse CD73 Antibody in functional ELISA. Immobilized Recombinant mouse CD73 Protein can bind mouse CD73 Antibody. |
| Alternate Names | 5'-nucleotidase; CD antigen CD73; eN; CALJA; CD73; EC 3.1.3.5; NT; Ecto-5'-nucleotidase; NT5; E5NT; 5'-NT; eNT; NTE |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 90% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | NT5E |
|---|---|
| Gene Full Name | 5'-nucleotidase, ecto (CD73) |
| Background | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] |
| Function | Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. [UniProt] |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. [UniProt] |
