ARG82070

Human Calprotectin ELISA Kit

Human Calprotectin ELISA Kit for ELISA and Human

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Overview

Product Description ARG82070 Human Calprotectin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Calprotectin in serum, plasma, milk, urine, saliva, CSF, cell lysate and cell culture supernatants.
Tested Reactivity Hu
Species Does Not React With Ms, Rat, Bov, Dog, Mk, Pig, Rb
Tested Application ELISA
Specificity Cross-Reactivity: Not react with Dog, Bovine, Monkey, Mouse, Rat, Pig and Rabbit Calprotectin. Not react with CD9, elastase, lactoferrin, lysozyme, myeloperoxidase, S100-A6, S100-A10, S100-A11, S100-A14, S100-A16 and S100-B.
Target Name Calprotectin
Conjugation Note Read at 450 nm.
Sensitivity 0.05 ng/ml
Sample Type Serum, plasma, milk, urine, saliva, CSF, cell lysate and cell culture supernatants.
Standard Range 0.078 - 5 ng/ml
Sample Volume 50 µl
Precision Intra-Assay CV: 3.9%; Inter-Assay CV: 8.4%
Alternate Names MA387; Leukocyte L1 complex light chain; 60B8AG; CFAG; p8; Urinary stone protein band A; P8; Calprotectin L1L subunit; NIF; S100 calcium-binding protein A8; MIF; MRP8; Calgranulin-A; Protein S100-A8; Migration inhibitory factor-related protein 8; CGLA; CAGA; L1Ag; Cystic fibrosis antigen; CP-10; MRP-8; S100A9; S100 calcium binding protein A9; MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387; S100A8/A9 complex; S100A8/A9 heterodimer complex

Application Instructions

Assay Time ~ 4.5 hours

Properties

Form 96 well
Storage Instruction Store components at 4°C or -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6279 Human S100A8

Swiss-port # P05109 Human Protein S100-A8

Gene Symbol S100A8; S100A9
Gene Full Name Calprotectin (S100A8/S100A9 complex)
Background The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq, Jul 2008]
Function S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. The iNOS-S100A8/A9 transnitrosylase complex directs selective inflammatory stimulus-dependent S-nitrosylation of GAPDH and probably multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity. [UniProt]
Cellular Localization Secreted. Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein. Note=Predominantly localized in the cytoplasm. Upon elevation of the intracellular calcium level, translocated from the cytoplasm to the cytoskeleton and the cell membrane. Upon neutrophil activation or endothelial adhesion of monocytes, is secreted via a microtubule-mediated, alternative pathway. [UniProt]
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Images (1) Click the Picture to Zoom In

  • ARG82070 Human Calprotectin ELISA Kit standard curve image

    ARG82070 Human Calprotectin ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

Associations of ultrasound-based inflammation patterns with peripheral innate lymphoid cell populations, serum cytokines/chemokines, and treatment response to methotrexate in rheumatoid arthritis and spondyloarthritis.

ELISA / Human / Serum

Manami Kato et al.
PLoS One.,  (2021)

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