ARG81144
Human Cardiac Troponin I ELISA Kit
Human Cardiac Troponin I ELISA Kit for ELISA and Human
Overview
| Product Description | ARG81144 Human Cardiac Troponin I ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cardiac Troponin I in serum. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Cardiac Troponin I |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 2.0 ng/ml |
| Sample Type | Serum |
| Standard Range | 2 - 75 ng/ml |
| Sample Volume | 100 µl |
| Alternate Names | RCM1; cTnI; Cardiac troponin I; TNNC1; CMD1FF; CMD2A; Troponin I, cardiac muscle; CMH7 |
Application Instructions
| Assay Time | ~ 2.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | TNNI3 |
| Gene Full Name | troponin I type 3 (cardiac) |
| Background | Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008] |
| Function | Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProt] |
| Highlight | Related products: Troponin antibodies; Troponin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). |
Images (1) Click the Picture to Zoom In
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ARG81144 Human Cardiac Troponin I ELISA Kit standard curve image
ARG81144 Human Cardiac Troponin I ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG81144 Human Cardiac Troponin I ELISA kit User manual |
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