Human FGF basic ELISA Kit

Human FGF basic ELISA Kit for ELISA and Human

Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Neuroscience kit; Signaling Transduction kit


Cat No Component Name Package Temp
ARG80141-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80141-002 Standard (Lyophilized) 3 X 4 ng/vial 4°C
ARG80141-003 Standard diluent buffer 20 ml 4°C
ARG80141-004 Antibody conjugate concentrate 400 µl 4°C
ARG80141-005 Antibody diluent buffer 16 ml 4°C
ARG80141-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80141-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80141-008 20X Wash buffer 50 ml 4°C
ARG80141-009 TMB substrate 12ml 4°C (Protect from light)
ARG80141-010 STOP solution 12ml 4°C
ARG80141-011 Plate sealer 4 strips Room temperature


Product Description ARG80141 Human FGF basic ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human FGF basic in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human FGF-5,6,9,10,16,17,18,19,20,21,23, KGF; mouse FGF-acidic,FGF-9
Target Name FGF basic
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 65 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 125 - 8000 pg/ml
Sample Volume 100 µl

CV: <10%

Alternate Names FGF-2; Fibroblast growth factor 2; bFGF; FGFB; Heparin-binding growth factor 2; BFGF; HBGF-2; Basic fibroblast growth factor

Application Instructions

Assay Time 4 hours


Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.


Gene Symbol FGF2
Gene Full Name fibroblast growth factor 2 (basic)
Background The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]
Function Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. [UniProt]
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Research Area Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Neuroscience kit; Signaling Transduction kit
PTM Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.

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  • ARG80141 Human FGF basic ELISA Kit standard curve image

    ARG80141 Human FGF basic ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG80141 Human FGF basic (bFGF) ELISA Kit User's manual download icon Download