ARG70607
Human GFAP recombinant protein (Active) (His-tagged, C-ter)
Human GFAP recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE and Human
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human GFAP recombinant protein |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | SDS-PAGE |
| Target Name | GFAP |
| Species | Human |
| A.A. Sequence | Met1 - Met432 |
| Expression System | E. coli |
| Activity | Active |
| Alternate Names | Glial fibrillary acidic protein; ALXDRD; GFAP |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GFAP |
| Gene Full Name | glial fibrillary acidic protein |
| Background | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| Function | GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt] |
| Cellular Localization | Cytoplasm. Note=Associated with intermediate filaments. [UniProt] |
| PTM | Phosphorylated by PKN1. [UniProt] |
