ARG70037

Human GM-CSF recombinant protein (Active) (His-tagged, N-ter)

Human GM-CSF recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE

Overview

Product Description E. coli expressed, His-tagged (N-ter) Active Human GM-CSF recombinant protein
Tested Application SDS-PAGE
Target Name GM-CSF
Species Human
A.A. Sequnce APARS PSPST QPWEH VNAIQ EARRL LNLSR DTAAE MNETV EVISE MFDLQ EPTCL QTRLE LYKQG LRGSL TKLKG PLTMM ASHYK QHCPP TPETS CATQI ITFES FKENL KDFLL VIPFD CWEPV QE with polyhistidine tag at the N-terminus.
Expression System E. coli
Activity Active
Activity Note Determined by its ability to induce TF-1 cells proliferation. The ED50 for this effect is < 0.5 ng/ml.
Alternate Names GM-CSF; Granulocyte-macrophage colony-stimulating factor; CSF; GMCSF; Molgramostin; Colony-stimulating factor; Sargramostim

Properties

Form Powder
Purification Ni-NTA chromatography.
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 98% (by SDS-PAGE)
Buffer PBS (pH 8.0)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CSF2
Gene Full Name colony stimulating factor 2 (granulocyte-macrophage)
Background The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]
Function Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. [UniProt]
Cellular Localization Secreted. [UniProt]
Highlight Related products:
GM-CSF antibodies; GM-CSF ELISA Kits; GM-CSF Duos / Panels; GM-CSF recombinant proteins;
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HMGB1 in inflammation
Inflammatory Cytokines

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  • ARG70037 Human GM-CSF recombinant protein (Active) (His-tagged, N-ter) SDS-PAGE image

    SDS-PAGE analysis of ARG70037 Human GM-CSF recombinant protein (Active) (His-tagged, N-ter).