ARG70788
Human GM2A recombinant protein (His-tagged, C-ter)
Human GM2A recombinant protein (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | HEK293 expressed, His-tagged (C-ter) Human GM2A recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | GM2A |
| Species | Human |
| A.A. Sequence | Met1 - Ile193 |
| Expression System | HEK293 |
| Alternate Names | Ganglioside GM2 activator; SAP-3; Cerebroside sulfate activator protein; GM2-AP; Sphingolipid activator protein 3 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | GM2A |
|---|---|
| Gene Full Name | GM2 ganglioside activator |
| Background | This gene encodes a small glycolipid transport protein which acts as a substrate specific co-factor for the lysosomal enzyme beta-hexosaminidase A. Beta-hexosaminidase A, together with GM2 ganglioside activator, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Mutations in this gene result in GM2-gangliosidosis type AB or the AB variant of Tay-Sachs disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009] |
| Function | The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity (By similarity). Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. [UniProt] |
| Cellular Localization | Lysosome. [UniProt] |
| PTM | The serines in positions 32 and 33 are absent in 80% of the sequenced protein. [UniProt] |
