ARG70795
Human Glypican 1 / GPC1 recombinant protein (Active) (His-tagged, C-ter)
Human Glypican 1 / GPC1 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | CHO expressed, His-tagged (C-ter) Active Human Glypican 1 / GPC1 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | Glypican 1 / GPC1 |
| Species | Human |
| A.A. Sequence | Met1 - Ser529 |
| Expression System | CHO |
| Activity | Active |
| Activity Note | Determinedd by its ability to bind human FGF-2 in functional ELISA. Immobilized Recombinant human FGF-2 Protein at 5 μg/mL (100 μL/well) can bind Recombinant Mouse Glypican 1 Protein. |
| Alternate Names | Glypican 1; GPC1; Glypican-1 [Cleaved into: Secreted glypican-1] |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% by SDS-PAGE gel and HPLC analyses. |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | GPC1 |
|---|---|
| Gene Full Name | Glypican 1 |
| Background | Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008] |
| Function | Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (By similarity). [UniProt] |
| Cellular Localization | Cell membrane; Endosome; Membrane; Secreted. [UniProt] |
| PTM | Disulfide bond; Glycoprotein; GPI-anchor; Heparan sulfate; Lipoprotein; Proteoglycan; S-nitrosylation. [UniProt] |
