Human Granzyme B ELISA Kit

Human Granzyme B ELISA Kit for ELISA and Human

Cell Biology and Cellular Response kit; Cell Death kit; Immune System kit
publication_link Publication3


Cat No Component Name Package Temp
ARG80171-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80171-002 Standard (Lyophilized) 3 X 1 ng/vial 4°C
ARG80171-003 Standard diluent buffer 20 ml 4°C
ARG80171-004 Antibody conjugate concentrate 400 µl 4°C
ARG80171-005 Antibody diluent buffer 16 ml 4°C
ARG80171-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80171-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80171-008 20X Wash buffer 50 ml 4°C
ARG80171-009 TMB substrate 12ml 4°C (Protect from light)
ARG80171-010 STOP solution 12ml 4°C
ARG80171-011 Plate sealer 4 strips Room temperature


Product Description ARG80171 Human Granzyme B ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Granzyme B in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with other soluble cytokine
Target Name Granzyme B
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 8 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl

CV: <10%

Alternate Names EC; CTLA-1; CSP-B; Granzyme B; CTLA1; CCPI; CGL-1; CGL1; Cytotoxic T-lymphocyte proteinase 2; T-cell serine protease 1-3E; HLP; CTSGL1; in-2; Cathepsin G-like 1; SECT; Granzyme-2; Human lymphocyte protein; C11; CSPB; Lymphocyte protease

Application Instructions

Assay Time 4 hours


Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 3002 Human GZMB

Swiss-port # P10144 Human Granzyme B

Gene Symbol GZMB
Gene Full Name granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Background Granzyme B is a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]
Function Granzyme B is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. [UniProt]
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Research Area Cell Biology and Cellular Response kit; Cell Death kit; Immune System kit

Images (1) Click the Picture to Zoom In

  • ARG80171 Human Granzyme B ELISA Kit standard curve image

    ARG80171 Human Granzyme B ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

B7-H3 inhibits the IFN-γ-dependent cytotoxicity of Vγ9Vδ2 T cells against colon cancer cells.

ELISA / Human / Cell culture supernatant

Lu Huimin et al.
OncoImmunology.,  (2020)




Tim-3 suppresses the killing effect of Vγ9Vδ2 T cells on colon cancer cells by reducing perforin and granzyme B expression.

ELISA / Human

Li Xiaomi et al.
Exp Cell Res.,  (2019)




The Effect of Tea Catechins on Natural Killer Cell Activity in the Elderly: A Pilot Study.

ELISA / Human

Iketani Ryo et al.
Japanese Journal of Clinical Pharmacology and Therapeutics.,  (2019)



Title Download Link
ARG80171 Human Granzyme B ELISA Kit User's manual download icon Download