ARG70042

Human IL1 alpha recombinant protein (Active) (His-tagged, C-ter)

Human IL1 alpha recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE

Overview

Product Description E. coli expressed, His-tagged (C-ter) Active Human IL1 alpha recombinant protein
Tested Application SDS-PAGE
Target Name IL1 alpha
Species Human
A.A. Sequnce MSAPF SFLSN VKYNF MRIIK YEFIL NDALN QSIIR ANDQY LTAAA LHNLD EAVKF DMGAY KSSKD DAKIT VILRI SKTQL YVTAQ DEDQP VLLKE MPEIP KTITG SETNL LFFWE THGTK NYFTS VAHPN LFIAT KQDYW VCLAG GPPSI TDFQI LENQA with polyhistidine tag at the C-terminus.
Expression System E. coli
Activity Active
Activity Note Determined by its ability to induce D10.G4.1 cells proliferation. The ED50 for this effect is < 10 pg/ml. The specific activity of recombinant human IL-1 alpha is approximately > 1 x 10^8 IU/mg.
Alternate Names IL-1 alpha; Interleukin-1 alpha; IL1; IL1F1; Hematopoietin-1; IL1-ALPHA; IL-1A

Properties

Form Powder
Purification Ni-NTA chromatography.
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 98% (by SDS-PAGE)
Buffer PBS (pH 8.0)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IL1A
Gene Full Name interleukin 1, alpha
Background The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]
Function Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. [UniProt]
Cellular Localization Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. [UniProt]
Highlight Related products:
IL1 alpha antibodies; IL1 alpha ELISA Kits; IL1 alpha recombinant proteins;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines

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  • ARG70042 Human IL1 alpha recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image

    SDS-PAGE analysis of ARG70042 Human IL1 alpha recombinant protein (Active) (His-tagged, C-ter).