ARG80113

Human IL10 ELISA Kit

Human IL10 ELISA Kit for ELISA and Human

Cancer kit; Immune System kit
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Component

Cat No Component Name Package Temp
ARG80113-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80113-002 Standard (Lyophilized) 3 X 0.2 ng/vial 4°C
ARG80113-003 Standard diluent buffer 20 ml 4°C
ARG80113-004 Antibody conjugate concentrate 400 µl 4°C
ARG80113-005 Antibody diluent buffer 16 ml 4°C
ARG80113-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80113-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80113-008 20X Wash buffer 50 ml 4°C
ARG80113-009 TMB substrate 12ml 4°C (Protect from light)
ARG80113-010 STOP solution 12ml 4°C
ARG80113-011 Plate sealer 4 strips Room temperature

Overview

Product Description

ARG80113 Human IL10 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL10 in Serum, Plasma, Cell culture supernatants.

Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IL-10 R alpha, IL-10 R beta/Fc Chimera, IL-22, IL-24
Target Name IL10
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 0.4 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 0.78 - 50 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Full Name interleukin 10
Alternate Names IL10A; GVHDS; TGIF; IL-10; Cytokine synthesis inhibitory factor; CSIF; Interleukin-10

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IL10
Gene Full Name interleukin 10
Background The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]
Function Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. [UniProt]
Highlight Related products:
IL10 antibodies; IL10 ELISA Kits;
Research Area Cancer kit; Immune System kit

Images (1) Click the Picture to Zoom In

  • ARG80113 Human IL10 ELISA Kit standard curve image

    ARG80113 Human IL10 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

Serum and peritoneal fluid levels of interleukin-6 and interleukin-37 as biomarkers for endometriosis.

ELISA / Human

Jiang Jianfa et al.
Gynecol Endocrinol.,  (2019)

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