ARG70682
Human IL15 recombinant protein (Active) (His-tagged, C-ter)
Human IL15 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | CHO expressed, His-tagged (C-ter) Active Human IL15 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL15 |
| Species | Human |
| A.A. Sequence | Asn49 - Ser162 |
| Expression System | CHO |
| Activity | Active |
| Activity Note | Determined by its ability to induce proliferation in CTLL-2 cells. The ED₅₀ for this effect is less than 0.8 μg/mL. |
| Alternate Names | IL-15; Interleukin-15 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | Il15 |
|---|---|
| Gene Full Name | interleukin 15 |
| Background | IL-15 is a member of the four alpha-helical bundle family of cytokines with a molecular weight of 14-15 kDa. Its in vitro activity is very similar to IL-2, except that IL-15 utilizes the IL-15 receptor alpha subunit for binding. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes. It also activates a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograpt rejection, and the pathogenesis of autoimmune diseases. |
| Function | Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. [UniProt] |
