ARG70761

Human IL2 recombinant protein (Active) (His-tagged, C-ter)

Human IL2 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE

Overview

Product Description CHO expressed, His-tagged (C-ter) Active Human IL2 recombinant protein
Tested Application SDS-PAGE
Target Name IL2
Species Human
A.A. Sequence Ala21 - Gln169
Expression System CHO
Activity Active
Activity Note Determined by its ability to induce proliferation in CTLL-2 cells. The ED₅₀ for this effect is less than 15 ng/mL.
Alternate Names TCGF; IL-2; lymphokine; Interleukin-2; Aldesleukin; T-cell growth factor

Properties

Form Powder
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IL2
Gene Full Name interleukin 2
Background Interleukin 2 (IL-2) is the cytokine secreted by T-cells immediately after the T cell receptors are activated by the binding of foreign antigens. The cytokine stimulates the growth, proliferation and differentiation of naïve T-cells into “effecter T-cells”, promotes the survival of antigen specific “memory T-cell”, and stimulates the maturation of “regulatory T-cells”. The cytokine is crucial for the development of adapted immunity, immune memory and the modulation of auto-immunity.
Function Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. [UniProt]