ARG80169

Human IL29 / IFN lambda ELISA Kit

Human IL29 / IFN lambda ELISA Kit for ELISA and Human

Immune System kit

Component

Cat No Component Name Package Temp
ARG80169-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80169-002 Standard (Lyophilized) 3 X 1 ng/vial 4°C
ARG80169-003 Standard diluent buffer 20 ml 4°C
ARG80169-004 Antibody conjugate concentrate 400 µl 4°C
ARG80169-005 Antibody diluent buffer 16 ml 4°C
ARG80169-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80169-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80169-008 20X Wash buffer 50 ml 4°C
ARG80169-009 TMB substrate 12ml 4°C (Protect from light)
ARG80169-010 STOP solution 12ml 4°C
ARG80169-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80169 Human IL29 / IFN lambda ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL29 / IFN lambda in Serum, Plasma, Cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IFN-αA/D, IFN-αA, IFN-αB2, IFN-αG, IFN-αI, IFN-β1a, IL-10 RβFc Chimera
Target Name IL29 / IFN lambda
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 15 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 31.25 - 2000 pg/ml
Sample Volume 100 µl
Precision CV: <10%
Alternate Names IFN-lambda-1; IL-29; Interferon lambda-1; Interleukin-29; Cytokine Zcyto21; IL29

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 282618 Human IFNL1

Swiss-port # Q8IU54 Human Interferon lambda-1

Gene Symbol IFNL1
Gene Full Name interferon, lambda 1
Background IL-29 is distantly related to type I interferons and the IL-10 family. Expression of IL-29 is induced by viral infection which interacts with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor beta (IL10RB) and interleukin 28 receptor alpha . IL-29 exhibits common features with type I IFNs such as antiviral activity, antiproliferative activity and in vivo antitumour activity. IL-29 acts similarly to IFNs, but is less effective generally and has activity in a more limited range of cell lines. IL-29 produced in response to viral infection, activates both monocytes and macrophages producing a restricted panel of cytokines and therefore is an important factor in activating innate immune responses at the site of viral infection. IFN-Lambda 1 antiviral and antiproliferative activity requires Interferon-Lambda 2 receptor tyrosine residues. Recombinant human IL-29 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 181 amino acids and having a molecular mass of?20 kDa.
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Research Area Immune System kit

Images (1) Click the Picture to Zoom In

  • ARG80169 Human IL29 / IFN lambda ELISA Kit standard curve image

    ARG80169 Human IL29 / IFN lambda ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG80169 Human IL29 / IFN lambda ELISA Kit User's manual download icon Download