ARG80110

Human IL6 ELISA Kit

Human IL6 ELISA Kit for ELISA and Human

Cancer kit; Immune System kit; Metabolism kit; Neuroscience kit
publication_link Publication1

Component

Cat No Component Name Package Temp
ARG80110-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80110-002 Standard (Lyophilized) 3 X 1 ng/vial 4°C
ARG80110-003 Standard diluent buffer 20 ml 4°C
ARG80110-004 Antibody conjugate concentrate 400 µl 4°C
ARG80110-005 Antibody diluent buffer 16 ml 4°C
ARG80110-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80110-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80110-008 20X Wash buffer 50 ml 4°C
ARG80110-009 TMB substrate 12ml 4°C (Protect from light)
ARG80110-010 STOP solution 12ml 4°C
ARG80110-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80110 Human IL6 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL6 in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human CNTF, G-CSF, sgp130, IL-6 sR, OSM, IL-11, IL-12, LIF, LIF R;mouse IL-6, IL-11, IL-12;rat CNTF
Target Name IL6
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 1.95 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 3.125 - 200 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Full Name interleukin 6
Alternate Names B-cell stimulatory factor 2; CDF; HSF; BSF-2; Interferon beta-2; IL-6; IFNB2; CTL differentiation factor; Interleukin-6; HGF; Hybridoma growth factor; BSF2; IFN-beta-2

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IL6
Gene Full Name interleukin 6
Background This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]
Function Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. [UniProt]
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Research Area Cancer kit; Immune System kit; Metabolism kit; Neuroscience kit
PTM N- and O-glycosylated.

Images (1) Click the Picture to Zoom In

  • ARG80110 Human IL6 ELISA Kit standard curve image

    ARG80110 Human IL6 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

Serum and peritoneal fluid levels of interleukin-6 and interleukin-37 as biomarkers for endometriosis.

ELISA / Human

Jiang Jianfa et al.
Gynecol Endocrinol.,  (2019)

publication_link

 

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