ARG70645
Human LIF recombinant protein (Active) (His-tagged, C-ter)
Human LIF recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human LIF recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | LIF |
| Species | Human |
| A.A. Sequence | Ser23 - Phe202 |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is less than 0.2 ng/mL. |
| Alternate Names | LIF; Leukemia inhibitory factor; Emfilermin; CDF; DIA; Differentiation-stimulating factor; MLPLI; HILDA; Melanoma-derived LPL inhibitor; D factor |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | LIF |
|---|---|
| Gene Full Name | leukemia inhibitory factor |
| Background | The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] |
| Function | LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. [UniProt] |
| Cellular Localization | Secreted. |
