ARG83757
Human Leptin ELISA Kit
Human Leptin ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83757 Human Leptin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Leptin in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Leptin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 50 µl |
| Precision | Intra-Assay CV: <10% Inter-Assay CV: <10% |
| Alternate Names | LEP; Leptin; OBS; OB; Leptin (Murine Obesity Homolog); Leptin (Obesity Homolog, Mouse); Obesity Factor; Obese Protein; Obese, Mouse, Homolog Of; LEPD |
Application Instructions
| Assay Time | 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 4°C, -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LEP |
| Gene Full Name | Leptin |
| Background | This gene encodes a protein that is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis. Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing. Mutations in this gene and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients. A mutation in this gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Aug 2017] |
| Function | Increases CD4+CD25- T-cell proliferation and reduces autophagy during TCR (T-cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation. [Uniprot] |
Images (1) Click the Picture to Zoom In
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ARG83757 Human Leptin ELISA Kit standard curve image
ARG83757 Human Leptin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83757 Human Leptin ELISA Kit User manual |
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