ARG80930

Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP1, TNF alpha)

Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP1, TNF alpha) for ELISA and Human

Overview

Product Description ARG80930 Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP-1, TNF alpha) is an Enzyme Immunoassay kit for the semi-quantification of GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP1 and TNF alpha in serum, plasma, cell culture supernatant and other biological samples.
Tested Reactivity Hu
Tested Application ELISA
Target Name M1/M2/MDSC Cytokine Multiplex
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sample Type Serum, plasma and cell culture supernatants.
Standard Range GM-CSF: 7.8 - 500 pg/ml
IFN gamma: 25 - 1600 pg/ml
IL4: 15.6 - 1000 pg/ml
IL6: 6.25 - 400 pg/ml
IL10: 25 - 1600 pg/ml
IL12: 15.6 - 1000 pg/ml
MCP1: 25 - 1600 pg/ml
TNF alpha: 15.6 - 1000 pg/ml
Sample Volume 100 µl /per well

Application Instructions

Assay Time 3.5 hour

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background GM-CSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]
IFN gamma is a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]
IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
IL6 is a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]
IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]
CCL2 (MCP-1) gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
Function GM-CSF is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. [UniProt]
IFN gamma is produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt]
IL4 participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. [UniProt]
IL6 is a cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. [UniProt]
IL10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. [UniProt]
MCP-1 is a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. [UniProt]
TNF alpha is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. [UniProt]

Images (9) Click the Picture to Zoom In

  • ARG80930 Antibodies Coating Pattern In Microtiter Plate

  • ARG80930 Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP1, TNF alpha) GM-CSF standard curve image

    ARG80930 Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP1, TNF alpha) results of a typical standard of GM-CSF run with optical density reading at 450 nm.

  • ARG80930 Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP1, TNF alpha) IFN gamma standard curve image

    ARG80930 Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP1, TNF alpha) results of a typical standard of IFN gamma run with optical density reading at 450 nm.

  • ARG80930 Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP1, TNF alpha) IL4 standard curve image

    ARG80930 Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP1, TNF alpha) results of a typical standard of IL4 run with optical density reading at 450 nm.

  • ARG80930 Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP1, TNF alpha) IL6 standard curve image

    ARG80930 Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP1, TNF alpha) results of a typical standard of IL6 run with optical density reading at 450 nm.

  • ARG80930 Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP-1, TNF alpha) IL10 standard curve image

    ARG80930 Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP-1, TNF alpha) results of a typical standard of IL10 run with optical density reading at 450 nm.

  • ARG80930 Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP-1, TNF alpha) IL12 standard curve image

    ARG80930 Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP-1, TNF alpha) results of a typical standard of IL12 run with optical density reading at 450 nm.

  • ARG80930 Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP1, TNF alpha) MCP1 standard curve image

    ARG80930 Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP1, TNF alpha) results of a typical standard of MCP1 run with optical density reading at 450 nm.

  • ARG80930 Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP1, TNF alpha) TNF alpha standard curve image

    ARG80930 Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP1, TNF alpha) results of a typical standard of TNF alpha run with optical density reading at 450 nm.

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