ARG70769
Human Mesothelin recombinant protein (Active) (His-tagged, C-ter)
Human Mesothelin recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | CHO expressed, His-tagged (C-ter) Active Human Mesothelin recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | Mesothelin |
| Species | Human |
| A.A. Sequence | Asp298 - Ser600 |
| Expression System | CHO |
| Activity | Active |
| Activity Note | Determinedd by its ability to bind human CA125 in functional ELISA. Immobilized Recombinant human CA125 Protein at 2 μg/mL (100 μL/well) can bind Recombinant Mouse MSLN Protein. |
| Alternate Names | MPF; Mesothelin; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor; SMRP |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 90% by SDS-PAGE gel and HPLC analyses |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | Msln |
|---|---|
| Gene Full Name | mesothelin |
| Background | This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010] |
| Function | Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. [UniProt] |
| Cellular Localization | Cell membrane, Golgi apparatus, Membrane, Secreted |
| PTM | Both MPF and the cleaved form of mesothelin are N-glycosylated. Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. [UniProt] |
