ARG70723

Human NCR3 / NKp30 recombinant protein (Active) (His-tagged, C-ter)

Human NCR3 / NKp30 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE

Overview

Product Description CHO expressed, His-tagged (C-ter) Active Human NCR3 / NKp30 recombinant protein
Tested Application SDS-PAGE
Target Name NCR3 / NKp30
Species Human
A.A. Sequence Leu19 - Gly135
Expression System CHO
Activity Active
Activity Note Determinedd by flow cytometry for its ability to bind a ligand expressed in K562 human chronic myelogenous leukemia cells.
Alternate Names NK-p30; 1C7; CD antigen CD337; Activating natural killer receptor p30; NKp30; Natural cytotoxicity triggering receptor 3; MALS; LY117; Natural killer cell p30-related protein; CD337

Properties

Form Powder
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 95% by SDS-PAGE gel and HPLC analyses.
Buffer PBS (pH 7.4)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol NCR3
Gene Full Name natural cytotoxicity triggering receptor 3
Background The protein encoded by this gene is a natural cytotoxicity receptor (NCR) that may aid NK cells in the lysis of tumor cells. The encoded protein interacts with CD3-zeta (CD247), a T-cell receptor. A single nucleotide polymorphism in the 5' untranslated region of this gene has been associated with mild malaria suceptibility. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]
Function Cytotoxicity-activating receptor that contributes to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. Engagement of NCR3 by BAG6 also promotes dendritic cell (DC) maturation, both through killing those DCs that did not properly acquire a mature phenotype, and inducing NK cells to release TNFA and IFNG, which promotes DC maturation. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]