ARG70698
Human Noggin recombinant protein (Active) (His-tagged, C-ter)
Human Noggin recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | CHO expressed, His-tagged (C-ter) Active Human Noggin recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | Noggin |
| Species | Human |
| A.A. Sequence | Gln28 - Cys232 |
| Expression System | CHO |
| Activity | Active |
| Activity Note | Determined by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is less than 0.05 µg/mL in the presence of 50 ng/mL of recombinant human BMP-4. |
| Alternate Names | SYM1; Noggin; SYNS1 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | NOG |
|---|---|
| Gene Full Name | noggin |
| Background | Essential for cartilage morphogenesis and joint formation.Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. |
| Function | Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Essential for cartilage morphogenesis and joint formation. Inhibits chondrocyte differentiation through its interaction with GDF5 (PubMed:21976273). [UniProt] |
| Cellular Localization | Secreted. |
