Human OPN / Osteopontin ELISA Kit

Human OPN / Osteopontin ELISA Kit for ELISA and Human

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Product Description ARG81268 Human OPN / Osteopontin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human OPN / Osteopontin in serum, plasma, urine or cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name OPN / Osteopontin
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm.

Sensitivity 32 pg/ml
Sample Type Serum, plasma, urine or cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Alternate Names BSPI; ETA-1; Uropontin; Osteopontin; Nephropontin; SPP-1; Bone sialoprotein 1; BNSP; Urinary stone protein; OPN; Secreted phosphoprotein 1

Application Instructions

Assay Time ~ 3.5 hours


Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 6696 Human SPP1

Swiss-port # P10451 Human Osteopontin

Gene Symbol SPP1
Gene Full Name secreted phosphoprotein 1
Background The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Function Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. [UniProt]
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PTM Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.
N- and O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.

Images (1) Click the Picture to Zoom In

  • ARG81268 Human OPN / Osteopontin ELISA Kit standard curve image

    ARG81268 Human OPN / Osteopontin ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

Transcriptomic analysis identifies upregulation of secreted phosphoprotein 1 in silicotic rats

ELISA / Human

Wenchen Cai et al.
Exp Ther Med.,  (2021)



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