Human PDGF BB ELISA Kit for ELISA and Human

Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Metabolism kit; Signaling Transduction kit


Cat No Component Name Package Temp
ARG80166-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80166-002 Standard (Lyophilized) 3 X 4 ng/vial 4°C
ARG80166-003 Standard diluent buffer 20 ml 4°C
ARG80166-004 Antibody conjugate concentrate 400 µl 4°C
ARG80166-005 Antibody diluent buffer 16 ml 4°C
ARG80166-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80166-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80166-008 20X Wash buffer 50 ml 4°C
ARG80166-009 TMB substrate 12ml 4°C (Protect from light)
ARG80166-010 STOP solution 12ml 4°C
ARG80166-011 Plate sealer 4 strips Room temperature


Product Description ARG80166 Human PDGF BB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PDGF BB in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human PDGF R alpha
Target Name PDGF BB
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 15.6 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 31.25 - 2000 pg/ml
Sample Volume 100 µl

CV: <10%

Alternate Names SIS; Becaplermin; Platelet-derived growth factor subunit B; IBGC5; PDGF subunit B; c-sis; SSV; PDGF-2; Proto-oncogene c-Sis; Platelet-derived growth factor beta polypeptide; PDGF2; Platelet-derived growth factor B chain

Application Instructions

Assay Time 4 hours


Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 5155 Human PDGFB

Swiss-port # P01127 Human Platelet-derived growth factor subunit B

Gene Symbol PDGFB
Gene Full Name platelet-derived growth factor beta polypeptide
Background The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]
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Research Area Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Metabolism kit; Signaling Transduction kit

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  • ARG80166 Human PDGF BB ELISA Kit standard curve image

    ARG80166 Human PDGF BB ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG80166 Human PDGF-BB ELISA Kit User's manual download icon Download