ARG80142

Human PLGF ELISA Kit

Human PLGF ELISA Kit for ELISA and Human

Cell Biology and Cellular Response kit

Component

Cat No Component Name Package Temp
ARG80142-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80142-002 Standard (Lyophilized) 2 X 2 ng/vial 4°C
ARG80142-003 Standard diluent buffer 20 ml 4°C
ARG80142-004 Antibody conjugate concentrate 400 µl 4°C
ARG80142-005 Antibody diluent buffer 16 ml 4°C
ARG80142-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80142-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80142-008 20X Wash buffer 50 ml 4°C
ARG80142-009 TMB substrate 12ml 4°C (Protect from light)
ARG80142-010 STOP solution 12ml 4°C
ARG80142-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80142 Human PLGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PLGF in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human EGF, PDGF-AA, PDGF-BB, VEGF121, VEGF165 and mouse VEGF165.
Target Name PLGF
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 15 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 31.25 - 2000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names PGFL; PlGF; PlGF-2; SHGC-10760; Placenta growth factor; D12S1900; PLGF

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5228 Human PGF

Swiss-port # P49763 Human Placenta growth factor

Gene Symbol PGF
Gene Full Name placental growth factor
Background This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011]
Function Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. [UniProt]
Cellular Localization Secreted. Note=The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. [UniProt]
Highlight Related products:
PLGF antibodies; PLGF ELISA Kits;
Related news:
The role of HDGF in tumor angiogenesis
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Cell Biology and Cellular Response kit
PTM N-glycosylated. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG80142 Human PLGF1 ELISA Kit standard curve image

    ARG80142 Human PLGF1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG80142 Human PLGF1 ELISA Kit User's manual download icon Download