Human Perforin ELISA Kit

Human Perforin ELISA Kit for ELISA and Human

Immune System kit
publication_link Publication6


Cat No Component Name Package Temp
ARG80173-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80173-002 Standard (Lyophilized) 2 X 2.5 ng/vial 4°C
ARG80173-003 Standard diluent buffer 20 ml 4°C
ARG80173-004 Antibody conjugate concentrate 400 µl 4°C
ARG80173-005 Antibody diluent buffer 16 ml 4°C
ARG80173-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80173-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80173-008 20X Wash buffer 50 ml 4°C
ARG80173-009 TMB substrate 12ml 4°C (Protect from light)
ARG80173-010 STOP solution 12ml 4°C
ARG80173-011 Plate sealer 4 strips Room temperature


Product Description ARG80173 Human Perforin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Perforin in Serum, Plasma, Cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IL-1 beta, IL-2, IL-3, IFN- gamma, TNF alpha, Fas, Fas-L, TRAIL
Target Name Perforin
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 20 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 39 - 2500 pg/ml
Sample Volume 100 µl

CV: <10%

Alternate Names FLH2; P1; PFN1; Cytolysin; HPLH2; PFP; Lymphocyte pore-forming protein; Perforin-1

Application Instructions

Assay Time 4 hours


Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 5551 Human PRF1

Swiss-port # P14222 Human Perforin-1

Gene Symbol PRF1
Gene Full Name perforin 1 (pore forming protein)
Background Perforin with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017]
Function Perforin plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. [UniProt]
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Research Area Immune System kit
PTM N-glycosylated.

Images (1) Click the Picture to Zoom In

  • ARG80173 Human Perforin ELISA Kit standard curve image

    ARG80173 Human Perforin ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

Redirecting natural killer cells to potentiate adoptive immunotherapy in solid tumors through stabilized Y-type bispecific aptamer

ELISA / Human / Cell culture supernatants

Youshi Zheng  et al.
Nanoscale.,  (2021)




Acute lymphoblastic leukemia-derived exosome inhibits cytotoxicity of natural killer cells by TGF-β signaling pathway

ELISA / Human / Cell culture supernatants

Huijun Yu et al.
3 Biotech.,  (2021)




B7-H3 inhibits the IFN-γ-dependent cytotoxicity of Vγ9Vδ2 T cells against colon cancer cells.

ELISA / Human / Cell culture supernatant

Lu Huimin et al.
OncoImmunology.,  (2020)




Tim-3 suppresses the killing effect of Vγ9Vδ2 T cells on colon cancer cells by reducing perforin and granzyme B expression.

ELISA / Human

Li Xiaomi et al.
Exp Cell Res.,  (2019)




The Effect of Tea Catechins on Natural Killer Cell Activity in the Elderly: A Pilot Study.

ELISA / Human

Iketani Ryo et al.
Japanese Journal of Clinical Pharmacology and Therapeutics.,  (2019)




Increase of Tumor Infiltrating γδ T-cells in Pancreatic Ductal Adenocarcinoma Through Remodeling of the Extracellular Matrix by a Hyaluronan Synthesis Suppressor, 4-Methylumbelliferone.

ELISA / Human

Suto Akiko et al.
Pancreas.,  (2019)



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