ARG83750
Human Perforin ELISA Kit
Human Perforin ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83750 Human Perforin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Perforin in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Perforin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 46.88 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 93.75 - 6000 pg/ml |
| Sample Volume | 50 µl |
| Precision | Intra-Assay CV: <10% Inter-Assay CV: <10% |
| Alternate Names | PRF1; Perforin 1; PFP; P1; HPLH2; Perforin 1 (Pore Forming Protein); Lymphocyte Pore-Forming Protein; Perforin-1; Cytolysin; Perforin 1 (Preforming Protein); Perforin |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 4°C, -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PRF1 |
| Gene Full Name | Perforin 1 |
| Background | This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017] |
| Function | Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu. [Uniprot] |
| PTM | Disulfide bond, Glycoprotein. [Uniprot] |
Images (1) Click the Picture to Zoom In
-
ARG83750 Human Perforin ELISA Kit standard curve image
ARG83750 Human Perforin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG83750 Human Perforin ELISA Kit User manual |
 Download
|
