ARG70730
Human Robo1 / DUTT1 recombinant protein (Active) (His-tagged, C-ter)
Human Robo1 / DUTT1 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | CHO expressed, His-tagged (C-ter) Active Human Robo1 / DUTT1 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | Robo1 / DUTT1 |
| Species | Human |
| A.A. Sequence | Gln26 - Pro897 |
| Expression System | CHO |
| Activity | Active |
| Activity Note | Determinedd by flow cytometry for its ability to bind a ligand expressed in HEK293 human embryonic kidney epithelial cell. |
| Alternate Names | Deleted in U twenty twenty; H-Robo-1; DUTT1; Roundabout homolog 1; SAX3 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | ROBO1 |
|---|---|
| Gene Full Name | Roundabout Guidance Receptor 1 |
| Background | Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| PTM | Ubiquitinated. May be deubiquitinated by USP33. |
