ARG70849
Human Sonic Hedgehog (C24II) recombinant protein (Active) (Tag free)
Human Sonic Hedgehog (C24II) recombinant protein (Active) (Tag free) for SDS-PAGE
Overview
| Product Description | HEK293 expressed, Human Sonic Hedgehog (C24II) recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | Sonic Hedgehog (C24II) |
| Species | Human |
| A.A. Sequence | Gly25 - Gly197 |
| Expression System | HEK293 |
| Activity | Active |
| Activity Note | Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.5-2 µg/mL. |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU per 1 μg of the protein by the LAL method. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
