ARG70644
Human TGF beta 3 recombinant protein (Active) (His-tagged, C-ter)
Human TGF beta 3 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human TGF beta 3 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | TGF beta 3 |
| Species | Human |
| A.A. Sequence | Ala301 - Ser412 |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to inhibit IL-4-induce proliferation in HT-2 cells. The ED₅₀ for this effect is less than 50 pg/mL. The specific activity of recombinant human TGF Beta 3 is approximately 2 x 10⁷ IU/mg. |
| Alternate Names | TGF-beta3; RNHF; LAP; ARVD; ARVD1; Transforming growth factor beta-3; TGF-beta-3 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | 20 mM sodium citrate and 0.2 M NaCl (pH 4.5) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | TGFB3 |
|---|---|
| Gene Full Name | transforming growth factor, beta 3 |
| Background | This gene encodes a member of the TGF-beta family of proteins. The encoded protein is secreted and is involved in embryogenesis and cell differentiation. Defects in this gene are a cause of familial arrhythmogenic right ventricular dysplasia 1. [provided by RefSeq, Mar 2009] |
| Function | Involved in embryogenesis and cell differentiation. [UniProt] |
| Cellular Localization | Cytoplasm |
| PTM | The precursor is cleaved into mature TGF-beta-3 and LAP, which remains non-covalently linked to mature TGF-beta-3 rendering it inactive. |
