ARG70681
Human TNF recombinant protein (Active) (His-tagged, C-ter)
Human TNF recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | CHO expressed, His-tagged (C-ter) Active Human TNF recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | TNF |
| Species | Human |
| A.A. Sequence | Leu80 - Leu235 |
| Expression System | CHO |
| Activity | Active |
| Activity Note | Determinedd by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D, with the ED50 being less than 50 pg/mL. |
| Alternate Names | Tumor necrosis factor ligand superfamily member 2; DIF; Cachectin; ICD2; ICD1; N-terminal fragment; TNF-a; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; NTF |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | TNF |
|---|---|
| Gene Full Name | tumor necrosis factor |
| Background | Tumor Necrosis Factor alpha (TNF alpha ) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Human TNF alpha is a 17.4 kDa protein containing 157 amino acid residues. |
