ARG70680

Human TNFSF11 recombinant protein (Active) (His-tagged, C-ter)

Human TNFSF11 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE

Overview

Product Description CHO expressed, His-tagged (C-ter) Active Human TNFSF11 recombinant protein
Tested Application SDS-PAGE
Target Name TNFSF11
Species Human
A.A. Sequence Lys158 - Asp316
Expression System CHO
Activity Active
Activity Note Determinedd by its ability to bind mouse RANKL Antibody in functional ELISA. Immobilized Recombinant mouse RANKL Protein can bind mouse RANKL Antibody.
Alternate Names TRANCE; Osteoprotegerin ligand; CD254; sOdf; Receptor activator of nuclear factor kappa-B ligand; OPTB2; RANKL; OPGL; Tumor necrosis factor ligand superfamily member 11; hRANKL2; TNF-related activation-induced cytokine; Osteoclast differentiation factor; ODF; CD antigen CD254

Properties

Form Powder
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol Tnfsf11
Gene Full Name tumor necrosis factor (ligand) superfamily, member 11
Background RANK (receptor activator of NF-kB) is a member of the TNFR family, identified as a dendritic cell membrane protein. Murine sRANK Ligand is a soluble 19.4 kDa protein, comprising the TNF-like extracellular domain of RANKL (174 amino acids). The full length of RANK Ligand is 39.6 kDa containing 36 amino acids.
Function Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. [UniProt]