Human VEGF ELISA Kit for ELISA and Human

Cancer kit; Cell Biology and Cellular Response kit; Metabolism kit; Signaling Transduction kit
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Cat No Component Name Package Temp
ARG80124-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80124-002 Standard (Lyophilized) 3 X 1 ng/vial 4°C
ARG80124-003 Standard diluent buffer 20 ml 4°C
ARG80124-004 Antibody conjugate concentrate 400 µl 4°C
ARG80124-005 Antibody diluent buffer 16 ml 4°C
ARG80124-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80124-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80124-008 20X Wash buffer 50 ml 4°C
ARG80124-009 TMB substrate 12ml 4°C (Protect from light)
ARG80124-010 STOP solution 12ml 4°C
ARG80124-011 Plate sealer 4 strips Room temperature


Product Description ARG80124 Human VEGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human VEGF in Serum, Plasma, Cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human VEGF-D
Target Name VEGF
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 8 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl

CV: <10%

Alternate Names MVCD1; Vascular permeability factor; VEGF-A; VPF; VEGF; Vascular endothelial growth factor A

Application Instructions

Assay Time 4 hours


Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 7422 Human VEGFA

Swiss-port # P15692 Human Vascular endothelial growth factor A

Gene Symbol VEGFA
Gene Full Name vascular endothelial growth factor A
Background This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. [provided by RefSeq, Jul 2008]
Highlight Related products:
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Research Area Cancer kit; Cell Biology and Cellular Response kit; Metabolism kit; Signaling Transduction kit

Images (1) Click the Picture to Zoom In

  • ARG80124 Human VEGF ELISA Kit standard curve image

    ARG80124 Human VEGF ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

Rotundic Acid Induces DNA Damage and Cell Death in Hepatocellular Carcinoma Through AKT/mTOR and MAPK Pathways.

ELISA / Human

Roy Gaurab et al.
Front. Oncol.,  (2019)



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ARG80124 Human VEGF ELISA Kit User's manual download icon Download