ARG70887
Human eIF4B recombinant protein (MBP-tagged, N-ter / His-tagged, C-ter)
Human eIF4B recombinant protein (MBP-tagged, N-ter / His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, Human MBP-tagged (N-ter) / His-tagged (C-ter) eIF4B recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | eIF4B |
| Species | Human |
| A.A. Sequence | Gln381 - Ala610 |
| Expression System | E. coli |
| Alternate Names | EIF-4B; eIF-4B; PRO1843; Eukaryotic translation initiation factor 4B |
Properties
| Form | Powder |
|---|---|
| Buffer | PBS |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration approximately 1 mg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C, protected from light and moisture, for up to 12 months. After reconstitution , aliquot and store at 2 to 8°C for up to 2 days, or at -20°C or -80°C for up to 3 months Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
Bioinformation
| Gene Symbol | EIF4B |
|---|---|
| Gene Full Name | eukaryotic translation initiation factor 4B |
| Background | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. |
| Function | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. [UniProt] |
| PTM | Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex. |
