ARG80130
Human sCD40L ELISA Kit
Human sCD40L ELISA Kit for ELISA and Human
Cell Biology and Cellular Response kit; Developmental Biology kit; Immune System kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80130-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80130-002 | Standard (Lyophilized) | 3 X 2 ng/vial | 4°C |
| ARG80130-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80130-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80130-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80130-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80130-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80130-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80130-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80130-010 | STOP solution | 12ml | 4°C |
| ARG80130-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80130 Human sCD40L ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human sCD40L in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human ANG, AR, BDNF, CD4, CD40, CNTF, CT-1, CTLA-4, Epo, Fas, CDNF, GITR, OPG, OSM |
| Target Name | sCD154 / sCD40L |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 30 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | TNFSF5; IMD3; T-cell antigen Gp39; HIGM1; CD40-L; gp39; CD40 ligand; Tumor necrosis factor ligand superfamily member 5; CD40L; CD154; TRAP; CD antigen CD154; hCD40L; IGM; T-BAM; TNF-related activation protein |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD40LG |
| Gene Full Name | CD40 ligand |
| Background | The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008] |
| Function | Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. [UniProt] |
| Highlight | Related products: CD154 antibodies; CD154 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cell Biology and Cellular Response kit; Developmental Biology kit; Immune System kit |
| PTM | The soluble form derives from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Not O-glycosylated. |
Images (1) Click the Picture to Zoom In
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ARG80130 Human sCD40L ELISA Kit standard curve image
ARG80130 Human sCD40L ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80130 Human sCD40L ELISA Kit User's manual |
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