ARG80125

Human sCD54 / sICAM1 ELISA Kit

Datasheet

Publication3

Ordering Information

Package

Price (USD)

96 wells

425.00

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Component

Cat No	Component Name	Package	Temp
ARG80125-001	Antibody-coated microplate	8 X 12 strips	4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80125-002	Standard (Lyophilized)	3 X 30 ng/vial	4°C
ARG80125-003	Standard diluent buffer	20 ml	4°C
ARG80125-004	Antibody conjugate concentrate	400 µl	4°C
ARG80125-005	Antibody diluent buffer	16 ml	4°C
ARG80125-006	HRP-Streptavidin concentrate	400 µl	4°C (Protect from light)
ARG80125-007	HRP-Streptavidin diluent buffer	16 ml	4°C
ARG80125-008	20X Wash buffer	50 ml	4°C
ARG80125-009	TMB substrate	12ml	4°C (Protect from light)
ARG80125-010	STOP solution	12ml	4°C
ARG80125-011	Plate sealer	4 strips	Room temperature

Overview

Product Description	ARG80125 Human sCD54 / sICAM1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human sCD54 / sICAM1 in serum, plasma and cell culture supernatants.
Tested Reactivity	Hu
Tested Application	ELISA
Specificity	No significant cross-reactivity or interference with Human sCD31, ICAM-2, sE-Selectin, sL-Selectin, sVCAM-1；mouse VCAM-1
Target Name	sCD54 / sICAM1
Conjugation	HRP
Conjugation Note	Substrate: TMB and read at 450 nm
Sensitivity	0.24 ng/ml
Sample Type	Serum, plasma and cell culture supernatants.
Standard Range	0.47 - 30 ng/ml
Sample Volume	100 µl
Precision	CV: <10%
Alternate Names	CD54; CD antigen CD54; BB2; P3.58; Intercellular adhesion molecule 1; Major group rhinovirus receptor; ICAM-1

Application Instructions

Assay Time	4 hours

Properties

Form	96 well
Storage Instruction	Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 3383 Human ICAM1 Swiss-port # P05362 Human Intercellular adhesion molecule 1
Gene Symbol	ICAM1
Gene Full Name	intercellular adhesion molecule 1
Background	This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
Function	ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity). [UniProt]
Highlight	Related products: CD54 antibodies; CD54 ELISA Kits; CD54 Duos / Panels; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData
Research Area	Cell Biology and Cellular Response kit; Developmental Biology kit; Immune System kit
PTM	Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

Images (1) Click the Picture to Zoom In

ARG80125 Human sCD54 / sICAM1 ELISA Kit standard curve image

ARG80125 Human sCD54 / sICAM1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

Prognostic value of serum levels of multiple adhesion factors in patients with sepsis-induced acute kidney injury

ELISA / Human / Serum

Yan Li et al.
Int Urol Nephrol, (2022)

Mechanism of Huashi Xingyu Qingre recipe in treating oral lichen planus based on network pharmacology and clinical trial verification

ELISA / Human / Serum

Deng Qianlan et al.
J Tradit Chin Med., (2022)

Testing feasibility of traditional Malay massage compared to relaxation for patients with non-speciﬁc low back pain: A pilot randomized-controlled trial.

ELISA / Human / Serum

Nurhanisah Sejari et al.
European Journal of Integrative Medicine., (2020)