Mouse CD169 / Siglec 1 ELISA Kit

Mouse CD169 / Siglec 1 ELISA Kit for ELISA and Mouse


Cat No Component Name Package Temp
ARG81881-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81881-002 Standard 2 X 10 ng/vial 4°C
ARG81881-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81881-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81881-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81881-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81881-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81881-008 25X Wash buffer 20 ml 4°C
ARG81881-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81881-010 STOP solution 10 ml (Ready to use) 4°C
ARG81881-011 Plate sealer 4 strips Room temperature


Product Description ARG81881 Mouse CD169 / Siglec 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD169 / Siglec 1 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD169 / Siglec 1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.5%; Inter-Assay CV: 7.6%
Alternate Names CD169; Siglec-1; dJ1009E24.1; Sialic acid-binding Ig-like lectin 1; SIGLEC-1; CD antigen CD169; SN; Sialoadhesin

Application Instructions

Assay Time ~ 5 hours


Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 20612 Mouse SIGLEC1

Swiss-port # Q62230 Mouse Sialoadhesin

Gene Symbol SIGLEC1
Gene Full Name sialic acid binding Ig-like lectin 1, sialoadhesin
Background This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008]
Function Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. [UniProt]
Highlight Related products:
CD169 antibodies; CD169 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

Images (1) Click the Picture to Zoom In

  • ARG81881 Mouse CD169 / Siglec 1 ELISA Kit standard curve image

    ARG81881 Mouse CD169 / Siglec 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81881 Mouse CD169 / Siglec 1 ELISA Kit User manual download icon Download