Mouse CD279 / PD-1 ELISA Kit

Mouse CD279 / PD-1 ELISA Kit for ELISA and Mouse


Cat No Component Name Package Temp
ARG81342-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81342-002 Standard (Lyophilized) 2 X 1.6 ng/vial 4°C
ARG81342-003 Standard diluent buffer 20 ml 4°C
ARG81342-004 Antibody conjugate concentrate 400 µl 4°C
ARG81342-005 Antibody diluent buffer 16 ml 4°C
ARG81342-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG81342-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG81342-008 20X Wash buffer 50 ml 4°C
ARG81342-009 TMB substrate 12 ml 4°C (Protect from light)
ARG81342-010 STOP solution 12 ml 4°C
ARG81342-011 Plate sealer 4 strips Room temperature


Product Description ARG81342 Mouse CD279 / PD-1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD279 / PD-1 in serum, plasma and cell culture supernatant.
Tested Reactivity Ms
Tested Application ELISA
Target Name CD279 / PD-1
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm.

Sensitivity 7.8 pg/ml
Sample Type Serum, plasma and cell culture supernatant.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision CV: <10%
Alternate Names hPD-l; CD279; PD-1; Protein PD-1; CD antigen CD279; PD1; hSLE1; SLEB2; Programmed cell death protein 1; hPD-1

Application Instructions

Assay Time ~ 3.5 hours


Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 18566 Mouse PDCD1

Swiss-port # Q02242 Mouse Programmed cell death protein 1

Gene Symbol PDCD1
Gene Full Name programmed cell death 1
Background CD279 / PD-1 is a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]
Function CD279 / PD-1 is an inhibitory receptor on antigen activated T-cells. It plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta.

The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311). The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552). [UniProt]
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  • ARG81342 Mouse CD279 / PD-1 ELISA Kit standard curve image

    ARG81342 Mouse CD279 / PD-1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
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